Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10923/865
Tipo: article
Título: Antibacterial activity of two MTA-based root canal sealers
Autor(es): Morgental, Renata Dornelles
Vier-Pelisser, Fabiana Vieira
Oliveira, Sílvia Dias de
Antunes, Fernanda C
Cogo, Deborah Meirelles
Kopper, Patricia Maria
Editor: Blackwell Scientific Publications
Fecha de Publicación: 2011
Volumen: 44
Número: 12
Primera página: 1128
Última página: 1133
Palabras clave: ANTIBACTERIAL ACTIVITY
ENDO CPM SEALER
ENDOFILL
MTA FILLAPEX
WHITE MTA
AGENTES ANTIBACTERIANOS
ODONTOLOGIA
Resumen: AIM: To evaluate the pH and antibacterial activity of Endo CPM Sealer and MTA Fillapex by two different methods, using white MTA and Endofill as references for comparison. METHODOLOGY: Antibacterial activity was evaluated against Enterococcus faecalis (ATCC 29212). The agar diffusion test (ADT) was performed to evaluate the effect before setting. The materials were placed in four equidistant wells made in ten agar plates. After incubation at 37 °C for 48 h, the inhibition zones were measured using a digital paquimeter. The direct contact test (DCT) was performed to assess the antibacterial effect after setting. Suspensions of crushed materials were prepared and mixed with E. faecalis. After different periods of time (1, 6, 15 and 60 min), the survival of bacteria was assessed by using 10-fold serial dilution and cultivated on agar plates in triplicate. Colony-forming units (CFU) mL(-1) were calculated after incubation. pH values were also measured in triplicate. Comparison between sealers in the ADT and DCT was performed by the Kruskal-Wallis test. RESULTS: In the ADT, inhibition zones were found with MTA Fillapex and Endofill. They were similar to each other and greater than the other sealers (P < 0.05). None of the tested sealers demonstrated antibacterial activity in the DCT, and thus, all sealers had similar bacterial counts compared with the negative control group (P > 0.05). White MTA and Endo CPM Sealer suspensions had pH values >11, whilst MTA Fillapex and Endofill had lower values. CONCLUSIONS: MTA Fillapex and Endofill had an antibacterial effect against E. faecalis before setting, but none of the sealers maintained antibacterial activity after setting, despite the high pH of the MTA-based materials.
URI: http://hdl.handle.net/10923/865
DOI: 10.1111/j.1365-2591.2011.01931.x
PMID: 21895702
ISSN: 0143-2885
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