Utilize este identificador para citar ou criar um atalho para este documento: http://hdl.handle.net/10923/893
Tipo: article
Título: Identification of Candida spp. by phenotypic tests and PCR
Autor(es): Marinho, Sandra Aparecida
Teixeira, Alice Becker
Santos, Otávio Silveira
Cazanova, Ricardo Flores
Ferreira, Carlos Alexandre Sanchez
Cherubini, Karen
Oliveira, Sílvia Dias de
Editora: Sociedade Brasileira de Microbiologia
Data de Publicação: 2010
Volume: 41
Página Inicial: 286
Página Final: 294
Palavras-chave: CANDIDA SPP
IDENTIFICATION
PCR
PHENOTYPIC TESTS
ODONTOLOGIA
PATOLOGIA BUCAL
CANDIDÍASE
Resumo: The correct identification of Candida species is of great importance, as it presents prognostic and therapeutical significance, allowing an early and appropriate antifungical therapy. The purpose of this study was to identify isolates of Candida spp. from oral mucosa of 38 patients with oral candidosis evaluated in 2004 by phenotypic methods and PCR, discriminating C. albicans from the other Candida species. The tests used for phenotypic analysis were germ-tube and chlamydoconidia production, culture in CHROMAgar™ Candida, carbohydrate assimilation test, growth at 45ºC and culture in Tween 80 agar. Genotypic confirmation was performed by PCR. Phenotypic tests showed that 63.2% strains formed germtubes, 73.7% produced chlamydoconidia, and 63.2% showed green colonies in chromogenic medium, presumptively indicating C. albicans or C. dubliniensis. The carbohydrate assimilation test confirmed these results. A total of 21% strains were identified as C. krusei and 13.2% were indicative of C. tropicalis. Of these later strains, three produced chlamydoconidia. The association of other phenotypic tests with culture in Tween 80 agar identified 95.8% of strains as C. albicans and 4.2% as C. dubliniensis. All 24 strains indicative of C. albicans and C. dubliniensis were confirmed by PCR as C. albicans.
URI: http://hdl.handle.net/10923/893
ISSN: 1517-8382
Aparece nas Coleções:Artigo de Periódico

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