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dc.contributor.authorMarinho, Sandra Aparecida-
dc.contributor.authorTeixeira, Alice Becker-
dc.contributor.authorSantos, Otávio Silveira-
dc.contributor.authorCazanova, Ricardo Flores-
dc.contributor.authorFerreira, Carlos Alexandre Sanchez-
dc.contributor.authorCherubini, Karen-
dc.contributor.authorOliveira, Sílvia Dias de-
dc.date.accessioned2012-04-25T13:46:20Z-
dc.date.available2012-04-25T13:46:20Z-
dc.date.issued2010-
dc.identifier.issn1517-8382en_US
dc.identifier.urihttp://hdl.handle.net/10923/893-
dc.description.abstractThe correct identification of Candida species is of great importance, as it presents prognostic and therapeutical significance, allowing an early and appropriate antifungical therapy. The purpose of this study was to identify isolates of Candida spp. from oral mucosa of 38 patients with oral candidosis evaluated in 2004 by phenotypic methods and PCR, discriminating C. albicans from the other Candida species. The tests used for phenotypic analysis were germ-tube and chlamydoconidia production, culture in CHROMAgar™ Candida, carbohydrate assimilation test, growth at 45ºC and culture in Tween 80 agar. Genotypic confirmation was performed by PCR. Phenotypic tests showed that 63.2% strains formed germtubes, 73.7% produced chlamydoconidia, and 63.2% showed green colonies in chromogenic medium, presumptively indicating C. albicans or C. dubliniensis. The carbohydrate assimilation test confirmed these results. A total of 21% strains were identified as C. krusei and 13.2% were indicative of C. tropicalis. Of these later strains, three produced chlamydoconidia. The association of other phenotypic tests with culture in Tween 80 agar identified 95.8% of strains as C. albicans and 4.2% as C. dubliniensis. All 24 strains indicative of C. albicans and C. dubliniensis were confirmed by PCR as C. albicans.en_US
dc.language.isoen_USen_US
dc.publisherSociedade Brasileira de Microbiologiaen_US
dc.subjectCANDIDA SPPen_US
dc.subjectIDENTIFICATIONen_US
dc.subjectPCRen_US
dc.subjectPHENOTYPIC TESTSen_US
dc.subjectODONTOLOGIAen_US
dc.subjectPATOLOGIA BUCALen_US
dc.subjectCANDIDÍASEen_US
dc.titleIdentification of Candida spp. by phenotypic tests and PCRen_US
dc.typearticleen_US
dc.publisher.placePorto Alegrept_BR
dc.jtitleBrazilian Journal of Microbiologyen_US
dc.publication.date2010en_US
dc.volume41en_US
dc.spage286en_US
dc.epage294en_US
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